DETEKSI INFEKSI Human palillomavirus (HPV) 16/18 PADA KARSINOMA SEL SKUAMOSA RONGGA MULUT DENGAN NESTED PCR

http://doi.org/10.22216/jit.2016.v10i1.394


Siti Hamidatul Aliyah(1*)

(1) STIKES Harapan Ibu Jambi
(*) Corresponding Author

Abstract


ABSTRACT

Head and neck cancer ranks fourth nationally cancer incidence in Indonesia. Oral SCC is one of Head and neck cancer incidence. Oral SCC related to several factors, including  smoking, alcohol, viral infection human papillomavirus (HPV 16/18) and genetic On the other hand, HPV E6 oncoprotein binds and inactivates TP53, and result in loss of control of the cell cycle. This study aimed to detect HPV 16/18 infection in oral SCC. Detection of HPV serotypes 16 and 18 performed on FFPE DNA isolates oral SCC with the method of nested Polymerase Chain Reaction (PCR). Nested PCR was performed in two stages, namely amplification with L1 primer, followed by specific PCR E6 HPV-16 and HPV-18. A total of 33% (11/33) FFPE samples showed positive for HPV 18 infection (single-sized DNA bands 415bp) and not detected the presence of infection with HPV 16. It can be concluded that the type of FFPE biosampel can be used for studies related to HPV infection. Furthermore, it should be tested on different types biosampel by a larger amount so as to represent the prevalence of oncogenic HPV infection in Indonesia.

Keyword: Oral SCC, HPV 16/18, PCR, FFPE

 

ABSTRAK

Kanker kepala dan leher menempati urutan kejadian kanker keempat di Indonesia. KSS rongga mulut merupakan salah satu kejadian kepala dan kanker leher. KSS rongga mulut terkait dengan beberapa faktor, termasuk merokok, alkohol, infeksi virus human papillomavirus (HPV 16/18) dan genetik Di sisi lain, HPV E6 mengikat onkoprotein dan menginaktivasi TP53, dan mengakibatkan hilangnya kontrol dari siklus sel. Penelitian ini bertujuan untuk mendeteksi HPV 16/18 infeksi pada KSS rongga mulut Deteksi HPV serotipe 16 dan 18 dilakukan pada FFPE DNA isolat SCC lisan dengan metode bersarang Polymerase Chain Reaction (PCR). Nested PCR dilakukan dalam dua tahap, yaitu amplifikasi dengan L1 primer, diikuti dengan PCR spesifik E6 HPV-16 dan HPV-18. Sebanyak 33% (11/33) sampel FFPE menunjukkan hasil positif untuk HPV 18 infeksi (pita DNA tunggal berukuran 415bp) dan tidak terdeteksi adanya infeksi HPV 16. Berdasarkan hasil tersebut dapat disimpulkan bahwa metode Nested PCR pada biosampel FFPE dapat digunakan untuk deteksi infeksi HPV pada KSS Rongga Mulut. Penelitian lebih lanjut perlu dilakukan pada jenis biosampel yang berbeda dengan jumlah yang lebih besar sehingga dapat mewakili prevalensi infeksi HPV onkogen di Indonesia.

Keyword: SCC Oral, HPV 16/18, PCR, FFPE


Keywords


SCC Oral, HPV 16/18, PCR, FFPE

Full Text:

PDF

References


Blot, W. J., McLaughlin, J. K., Winn, D. M., Austin, D. F., Greenberg, R. S., Preston-Martin, S., … Fraumeni, J. F. (1988). Smoking and drinking in relation to oral and pharyngeal cancer. Cancer Research, 48(11), 3282–3287.

Bodaghi, S., Wood, L. V., Roby, G., Ryder, C., Steinberg, S. M., & Zheng, Z. M. (2005). Could human papillomaviruses be spread through blood? Journal of Clinical Microbiology, 43(11), 5428–5434.

Carter, J. J., Madeleine, M. M., Shera, K., Schwartz, S. M., Cushing-Haugen, K. L., Wipf, G. C., … Galloway, D. A. (2001). Human papillomavirus 16 and 18 L1 serology compared across anogenital cancer sites. Cancer Research, 61(5), 1934–1940.

Chen, A. C.-H., Waterboer, T., Keleher, A., Morrison, B., Jindal, S., McMillan, D., … Antonsson, A. (2011). Human papillomavirus in benign prostatic hyperplasia and prostatic adenocarcinoma patients. Pathology Oncology Research : POR, 17(3), 613–7.

Chin, D., Boyle, G. M., Theile, D. R., Parsons, P. G., & Coman, W. B. (2004). Molecular introduction to head and neck cancer (HNSCC) carcinogenesis. British Journal of Plastic Surgery, 57(7), 595–602.

D’Souza, G., Agrawal, Y., Halpern, J., Bodison, S., & Gillison, M. L. (2009). Oral sexual behaviors associated with prevalent oral human papillomavirus infection. The Journal of Infectious Diseases, 199(9), 1263–1269.

D’Souza, G., Kreimer, A. R., Viscidi, R., Pawlita, M., Fakhry, C., Koch, W. M., … Gillison, M. L. (2007). Case-control study of human papillomavirus and oropharyngeal cancer. The New England Journal of Medicine, 356(19), 1944–1956.

Gasco, M., & Crook, T. (2003). The p53 network in head and neck cancer. Oral Oncology. http://doi.org/10.1016/S1368-8375(02)00163-X

Kabat, G. C., Chang, C. J., & Wynder, E. L. (1994). The role of tobacco, alcohol use, and body mass index in oral and pharyngeal cancer. International Journal of Epidemiology, 23(6), 1137–1144.

Lajer, C. B., & von Buchwald, C. (2010). The role of human papillomavirus in head and neck cancer. APMIS : Acta Pathologica, Microbiologica, et Immunologica Scandinavica, 118(6-7), 510–519.

Lam, K. Y., He, D., Ma, L., Zhang, D., Ngan, H. Y., Wan, T. S., & Tsao, S. W. (1997). Presence of human papillomavirus in esophageal squamous cell carcinomas of Hong Kong Chinese and its relationship with p53 gene mutation. Hum Pathol, 28(6), 657–663. R

Leemans, C. R., Braakhuis, B. J. M., & Brakenhoff, R. H. (2011). The molecular biology of head and neck cancer. Nature Reviews. Cancer, 11(1), 9–22.

Manos, M. M., Waldman, J., Zhang, T. Y., Greer, C. E., Eichinger, G., Schiffman, M. H., & Wheeler, C. M. (1994). Epidemiology and partial nucleotide sequence of four novel genital human papillomaviruses. The Journal of Infectious Diseases, 170(5), 1096–1099.

Marur, S., D’Souza, G., Westra, W. H., & Forastiere, A. A. (2010). HPV-associated head and neck cancer: A virus-related cancer epidemic. The Lancet Oncology. http://doi.org/10.1016/S1470-2045(10)70017-6

McKaig, R. G., Baric, R. S., & Olshan, A. F. (1998). Human papillomavirus and head and neck cancer: epidemiology and molecular biology. Head & Neck, 20(3), 250–265.

Sankaranarayanan, R., Nair, M. K., Mathew, B., Balaram, P., Sebastian, P., & Dutt, S. C. (1992). Recent results of oral cancer research in Kerala, India. Head and Neck, 14(2), 107–112.

Shen, H., Zheng, Y., Sturgis, E. M., Spitz, M. R., & Wei, Q. (2002). P53 codon 72 polymorphism and risk of squamous cell carcinoma of the head and neck: A case-control study. Cancer Letters, 183(2), 123–130.

Wang, Z., Gerstein, M., & Snyder, M. (2009). RNA-Seq: a revolutionary tool for transcriptomics. Nature Reviews. Genetics, 10(1), 57–63.


Refbacks

  • There are currently no refbacks.


Copyright (c) 2016 Jurnal Ipteks Terapan

Online ISSN : 2460-5611 | Print ISSN : 1979-9292

Publish by LLDIKTI Wilayah X (Sumatera Barat, Riau, Jambi dan Kepulauan Riau)

Jl. Khatib Sulaiman No 1 Kota Padang. Kode Pos 25144. Telp 0751-7056737. Fax 0751-7056737. Website:http://www.kopertis10.or.id

Web Analytics Made Easy - StatCounter View My Stats

Creative Commons License 

This work is licensed under a Creative Commons Attribution 4.0 International License.